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Figure 2. Cytotoxic effect of compounds T1023 and T1084 in vitro according to the MTT-test in relation to human malignant and normal cells. (A–G) Representative graphs of survival of human melanoma A375 cells (A), human mammary ductal adenocarcinoma MFC-7 cells (B), human chronic myelogenous leukemia K562 cells (C), human cervical cancer HeLa cells (D), human ovarian carcinoma OAW42 cells (E), human prostate cancer PC-3 cells (F), and a normal human fetal lung <t>fibroblast</t> MRC-5 cells (G) after 72 h of cell growth in the presence of increasing concentrations of T1023 and T1084. IC50 values were determined as M ± SD from four independent experiments. # significant difference (p = 0.01764) between the IC50 values for T1023 and T1084. (H) Sensitivity indexes of malignant cell lines to toxic effects of T1023 and T1084, calculated as the ratio of the IC50 value for MRC-5 and the IC50 value for the corresponding cell line.
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Figure 2. Cytotoxic effect of compounds T1023 and T1084 in vitro according to the MTT-test in relation to human malignant and normal cells. (A–G) Representative graphs of survival of human melanoma A375 cells (A), human mammary ductal adenocarcinoma MFC-7 cells (B), human chronic myelogenous leukemia K562 cells (C), human cervical cancer HeLa cells (D), human ovarian carcinoma OAW42 cells (E), human prostate cancer PC-3 cells (F), and a normal human fetal lung <t>fibroblast</t> MRC-5 cells (G) after 72 h of cell growth in the presence of increasing concentrations of T1023 and T1084. IC50 values were determined as M ± SD from four independent experiments. # significant difference (p = 0.01764) between the IC50 values for T1023 and T1084. (H) Sensitivity indexes of malignant cell lines to toxic effects of T1023 and T1084, calculated as the ratio of the IC50 value for MRC-5 and the IC50 value for the corresponding cell line.
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Figure 2. Cytotoxic effect of compounds T1023 and T1084 in vitro according to the MTT-test in relation to human malignant and normal cells. (A–G) Representative graphs of survival of human melanoma A375 cells (A), human mammary ductal adenocarcinoma MFC-7 cells (B), human chronic myelogenous leukemia K562 cells (C), human cervical cancer HeLa cells (D), human ovarian carcinoma OAW42 cells (E), human prostate cancer PC-3 cells (F), and a normal human fetal lung <t>fibroblast</t> MRC-5 cells (G) after 72 h of cell growth in the presence of increasing concentrations of T1023 and T1084. IC50 values were determined as M ± SD from four independent experiments. # significant difference (p = 0.01764) between the IC50 values for T1023 and T1084. (H) Sensitivity indexes of malignant cell lines to toxic effects of T1023 and T1084, calculated as the ratio of the IC50 value for MRC-5 and the IC50 value for the corresponding cell line.
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Figure 2. Cytotoxic effect of compounds T1023 and T1084 in vitro according to the MTT-test in relation to human malignant and normal cells. (A–G) Representative graphs of survival of human melanoma A375 cells (A), human mammary ductal adenocarcinoma MFC-7 cells (B), human chronic myelogenous leukemia K562 cells (C), human cervical cancer HeLa cells (D), human ovarian carcinoma OAW42 cells (E), human prostate cancer PC-3 cells (F), and a normal human fetal lung fibroblast MRC-5 cells (G) after 72 h of cell growth in the presence of increasing concentrations of T1023 and T1084. IC50 values were determined as M ± SD from four independent experiments. # significant difference (p = 0.01764) between the IC50 values for T1023 and T1084. (H) Sensitivity indexes of malignant cell lines to toxic effects of T1023 and T1084, calculated as the ratio of the IC50 value for MRC-5 and the IC50 value for the corresponding cell line.

Journal: International journal of molecular sciences

Article Title: In Vitro Cytotoxic Potential and In Vivo Antitumor Effects of NOS/PDK-Inhibitor T1084.

doi: 10.3390/ijms25179711

Figure Lengend Snippet: Figure 2. Cytotoxic effect of compounds T1023 and T1084 in vitro according to the MTT-test in relation to human malignant and normal cells. (A–G) Representative graphs of survival of human melanoma A375 cells (A), human mammary ductal adenocarcinoma MFC-7 cells (B), human chronic myelogenous leukemia K562 cells (C), human cervical cancer HeLa cells (D), human ovarian carcinoma OAW42 cells (E), human prostate cancer PC-3 cells (F), and a normal human fetal lung fibroblast MRC-5 cells (G) after 72 h of cell growth in the presence of increasing concentrations of T1023 and T1084. IC50 values were determined as M ± SD from four independent experiments. # significant difference (p = 0.01764) between the IC50 values for T1023 and T1084. (H) Sensitivity indexes of malignant cell lines to toxic effects of T1023 and T1084, calculated as the ratio of the IC50 value for MRC-5 and the IC50 value for the corresponding cell line.

Article Snippet: Cell Lines A normal human fetal lung fibroblast cell line (MRC-5), human melanoma cell line (A375), human cervix adenocarcinoma cell line (HeLa), human mammary ductal adenocarcinoma cell line (MFC-7), human chronic myelogenous leukemia cell line (K562), human prostatic adenocarcinoma cell line (PC-3) (American Type Culture Collection, Manassas, VA, USA), and a human ovarian carcinoma cell line (OAW42) (Federal Scientific and Clinical Center of Federal Biomedical Agency of Russia, Moscow) were grown in RPMI-1640 medium (PanEco, Ltd., Moscow, Russia).

Techniques: In Vitro